/usr/bin/needle -asequence /home/mercutio22/Dropbox/My_scripts/Fabi/resultados/referencia.fasta -bsequence /home/mercutio22/Dropbox/My_scripts/Fabi/resultados/118856_3450F_E1.fasta -gapopen 10 -gapextend 0.5 -outfile /home/mercutio22/Dropbox/My_scripts/Fabi/resultados/118856_3450F_E1_aligned.txt
['/usr/bin/needle', '-asequence', '/home/mercutio22/Dropbox/My_scripts/Fabi/resultados/referencia.fasta', '-bsequence', '/home/mercutio22/Dropbox/My_scripts/Fabi/resultados/118856_3450F_E1.fasta', '-gapopen', '10', '-gapextend', '0.5', '-outfile', '/home/mercutio22/Dropbox/My_scripts/Fabi/resultados/118856_3450F_E1_aligned.txt']
Traceback (most recent call last):
  File "louise.py", line 67, in <module>
    processar_lote(pasta, referencia)
  File "louise.py", line 62, in processar_lote
    needle_run = subprocess.call(needle_cline)
  File "/usr/lib/python2.6/subprocess.py", line 470, in call
    return Popen(*popenargs, **kwargs).wait()
  File "/usr/lib/python2.6/subprocess.py", line 623, in __init__
    errread, errwrite)
  File "/usr/lib/python2.6/subprocess.py", line 1141, in _execute_child
    raise child_exception
OSError: [Errno 2] No such file or directory